Description
Applications：
LAMBDA MINIFOR laboratory fermentor and bioreactor
The LAMBDA MINIFOR bench-top autoclavable laboratory fermenter and bioreactor was developed as a result of the need to construct a small laboratory fermentor for working volume ranges from 35 ml to over 6 liters.
Based on long personal practical experience in fermentation and cell culture, a high quality fermentor-bioreactor which is easy to use and with the capacity to measure and control all the important parameters of the biological culture, has been created (which required new ideas and many innovations).
Smallest footprint for parallel experiments
The fermentor had to take up minimum space on the bench, but with good access to all parts. Several fermentors should, when placed side by side, be suitable for the optimization of growth parameters of the culture or optimization of bio-transformations, etc. (highly suitable for parallel running experiments).
The space requirement of the LAMBDA MINIFOR fermentor-bioreactor with working vessel and the control unit is: 22 cm x 38 cm x 40 cm (W x H x D).
Technical Specifications
Power: Universal power supply for mains 100-245 V AC/50-60Hz, 560W, CE conform
Dimensions: 22 x 40 x 38 cm (W x D x H)
Display: LCD 4 x 40 digits with backlight illumination
Fermentor vessel: Pyrex glass with 6 to 8 side necks; 0.3, 0.4, 1, 3, 7 liter vessels
Temperature control: High efficiency 150 W infrared (IR) radiation heat source with gilded parabolic reflector
Regulation: from 5°C over RT to 70°C
Measurement: from 0 to 99.9°C in 0.1°C steps
Precision: +/- 0.2°C (0 to 60°C)
Sensor: Pt 100 incorporated in the glass electrode of the pH probe
pH control: sterilisable pH electrode pH 0-14 with automatic temperature correction, two-point semiautomatic calibration and Variopin connector
Resolution: 0.01 pH unit
Precision: +/- 0.02 pH unit
pO2 control: sterilisable Clark type oxygen sensor with fast response, automatic temperature correction, two-point semiautomatic calibration, dissolved oxygen (DO) control through regulation of the airflow rate
Range: 0 to 25 mg oxygen/ l, in 0.1 mg/l steps
Air flow: 0 to 5 l/min in 0.01 l/min steps, measured by precise mass flow meter, linearity +/- 3%, reproducibility +/- 0.5%
Control: proportional valve controlled by microprocessor
For supplied air pressure: 0.05 – 0.2 MPa (0.5 – 2 atm)
Agitation: 50 W Vibromixer 0 to 20 Hz (0 to 1200 rpm) in 0.1 Hz steps (6 rpm) with 1 or more stirring discs; Sterility similar to magnetic coupling
Selectable parameter ‘X’: an additional parameter can be controlled by the instrument (foaming control, weight (for continuous cultures), pCO2, redox potential, conductivity, optical density, etc.); with standard 0-10V or 0-20mA output
Ports / side necks: One large quadruple sampling or additions port with four needles with LAMBDA PEEK double-seal connections, used for sampling, inoculation, antifoam, feeds, harvest, addition of correction solutions etc., additional double ports are available.
Pumps: up to 4 independent pumps (PRECIFLOW, MULTIFLOW, HIFLOW or MAXIFLOW) with speed variation from 0 to 100 % can be used with MINIFOR lab fermenter-bioreactor
Gas flow control: In addition to pumps, several electronic flow controllers with flow rate ranges of 0-5 l/min (MASSFLOW 5000) or 0-500 ml/min (MASSFLOW 500) can be used for the controlled addition of gases (e.g. N2, O2, air, CO2) in cell cultures; freely configurable gas station module
Working temperature: 0 – 40 °C
Working humidity: 0 – 90 % RH, not condensing
Weight: 7.5 kg
PC control: complete PC control and data processing using the fermentation software FNet (for up to 6 MINIFOR fermenters) or SIAM (for an even higher number of instruments)
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